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anti irf3 202  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti irf3 202
    Anti Irf3 202, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+irf3+202/IRF-3+Rabbit+mAb/10__1128_slash_jvi__01251___17-85-61-65
    Average 96 stars, based on 1249 article reviews
    anti irf3 202 - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Genotype 2 Strains of Porcine Reproductive and Respiratory Syndrome Virus Dysregulate Alveolar Macrophage Cytokine Production via the Unfolded Protein Response
    Article Snippet: Equivalent protein amounts of each extract (25 to 196 60 μg per well) were subjected to separation in a 10% SDS-PAGE gel and subsequently 197 transferred onto a 0.2μ PVDF membrane (Bio-RAD, Hercules, CA, USA) for Western blot 198 analysis. .. Membranes were incubated in blocking buffer [2% fish gelatin in TBST solution (50 199 mM Tris, pH 7.5, 500 mM NaCl and 0.5 % Tween 20)] at RT for 1 h. Afterward, the membranes 200 were incubated at 4°C overnight with one of the following unconjugated primary antibodies (Ab) 201 (1:1000 dilution of manufacturer’s original concentration in TBST with 5% BSA): anti-IRF3 202 (clone D83B9, Cell Signaling, Danvers, MA, USA), anti-phospho-IRF3 (Ser396) (clone 4D4G, 203 Cell Signaling), anti-NF-κB p65 (#3034, Cell Signaling), anti-NF-κB p65 (#Sc109, Santa Cruz 204 on O ctober 27, 2017 by U N IV O F N E W C A S T LE http://jvi.asm .org/ D ow nloaded from 12 Biotechnology, Santa Cruz, CA, USA), anti-phospho NF-κB p65 (Ser536) (clone 93H1, Cell 205 Signaling), anti-STAT1 (sc346,), anti-phospho STAT1 (Tyr701) (SC-7988, Santa Cruz 206 Biotechnology), anti-eIF2 (#9722, Cell Signaling), anti-phospho-eIF2 (#9721, Cell Signaling), 207 anti-CHOP (clone L63F7, Cell Signaling), or anti-ß-actin (#4967, Cell Signaling). .. Membranes 208 were then washed four times in TBST solution, and incubated with horseradish peroxidase 209 (HRP)-conjugated anti-rabbit immunoglobulin (IgG) secondary Ab (sc2004, Santa Cruz 210 Biotechnology, Santa Cruz, CA, USA) (1: 8000 in blocking buffer) at RT for 1 h. After being 211 washed again 4 times with TBST, the membranes were incubated with a chemiluminescence 212 reagent (GE Healthcare, Little Chalfont, Buckinghamshire, UK) to enable detection of bound 213 secondary Ab.

    Blocking Assay:

    Article Title: Genotype 2 Strains of Porcine Reproductive and Respiratory Syndrome Virus Dysregulate Alveolar Macrophage Cytokine Production via the Unfolded Protein Response
    Article Snippet: Equivalent protein amounts of each extract (25 to 196 60 μg per well) were subjected to separation in a 10% SDS-PAGE gel and subsequently 197 transferred onto a 0.2μ PVDF membrane (Bio-RAD, Hercules, CA, USA) for Western blot 198 analysis. .. Membranes were incubated in blocking buffer [2% fish gelatin in TBST solution (50 199 mM Tris, pH 7.5, 500 mM NaCl and 0.5 % Tween 20)] at RT for 1 h. Afterward, the membranes 200 were incubated at 4°C overnight with one of the following unconjugated primary antibodies (Ab) 201 (1:1000 dilution of manufacturer’s original concentration in TBST with 5% BSA): anti-IRF3 202 (clone D83B9, Cell Signaling, Danvers, MA, USA), anti-phospho-IRF3 (Ser396) (clone 4D4G, 203 Cell Signaling), anti-NF-κB p65 (#3034, Cell Signaling), anti-NF-κB p65 (#Sc109, Santa Cruz 204 on O ctober 27, 2017 by U N IV O F N E W C A S T LE http://jvi.asm .org/ D ow nloaded from 12 Biotechnology, Santa Cruz, CA, USA), anti-phospho NF-κB p65 (Ser536) (clone 93H1, Cell 205 Signaling), anti-STAT1 (sc346,), anti-phospho STAT1 (Tyr701) (SC-7988, Santa Cruz 206 Biotechnology), anti-eIF2 (#9722, Cell Signaling), anti-phospho-eIF2 (#9721, Cell Signaling), 207 anti-CHOP (clone L63F7, Cell Signaling), or anti-ß-actin (#4967, Cell Signaling). .. Membranes 208 were then washed four times in TBST solution, and incubated with horseradish peroxidase 209 (HRP)-conjugated anti-rabbit immunoglobulin (IgG) secondary Ab (sc2004, Santa Cruz 210 Biotechnology, Santa Cruz, CA, USA) (1: 8000 in blocking buffer) at RT for 1 h. After being 211 washed again 4 times with TBST, the membranes were incubated with a chemiluminescence 212 reagent (GE Healthcare, Little Chalfont, Buckinghamshire, UK) to enable detection of bound 213 secondary Ab.

    Concentration Assay:

    Article Title: Genotype 2 Strains of Porcine Reproductive and Respiratory Syndrome Virus Dysregulate Alveolar Macrophage Cytokine Production via the Unfolded Protein Response
    Article Snippet: Equivalent protein amounts of each extract (25 to 196 60 μg per well) were subjected to separation in a 10% SDS-PAGE gel and subsequently 197 transferred onto a 0.2μ PVDF membrane (Bio-RAD, Hercules, CA, USA) for Western blot 198 analysis. .. Membranes were incubated in blocking buffer [2% fish gelatin in TBST solution (50 199 mM Tris, pH 7.5, 500 mM NaCl and 0.5 % Tween 20)] at RT for 1 h. Afterward, the membranes 200 were incubated at 4°C overnight with one of the following unconjugated primary antibodies (Ab) 201 (1:1000 dilution of manufacturer’s original concentration in TBST with 5% BSA): anti-IRF3 202 (clone D83B9, Cell Signaling, Danvers, MA, USA), anti-phospho-IRF3 (Ser396) (clone 4D4G, 203 Cell Signaling), anti-NF-κB p65 (#3034, Cell Signaling), anti-NF-κB p65 (#Sc109, Santa Cruz 204 on O ctober 27, 2017 by U N IV O F N E W C A S T LE http://jvi.asm .org/ D ow nloaded from 12 Biotechnology, Santa Cruz, CA, USA), anti-phospho NF-κB p65 (Ser536) (clone 93H1, Cell 205 Signaling), anti-STAT1 (sc346,), anti-phospho STAT1 (Tyr701) (SC-7988, Santa Cruz 206 Biotechnology), anti-eIF2 (#9722, Cell Signaling), anti-phospho-eIF2 (#9721, Cell Signaling), 207 anti-CHOP (clone L63F7, Cell Signaling), or anti-ß-actin (#4967, Cell Signaling). .. Membranes 208 were then washed four times in TBST solution, and incubated with horseradish peroxidase 209 (HRP)-conjugated anti-rabbit immunoglobulin (IgG) secondary Ab (sc2004, Santa Cruz 210 Biotechnology, Santa Cruz, CA, USA) (1: 8000 in blocking buffer) at RT for 1 h. After being 211 washed again 4 times with TBST, the membranes were incubated with a chemiluminescence 212 reagent (GE Healthcare, Little Chalfont, Buckinghamshire, UK) to enable detection of bound 213 secondary Ab.



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    Cell Signaling Technology Inc anti irf3 202
    Anti Irf3 202, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+irf3+202/IRF-3+Rabbit+mAb/10__1128_slash_jvi__01251___17-85-61-65
    Average 96 stars, based on 1 article reviews
    anti irf3 202 - by Bioz Stars, 2026-09
    96/100 stars
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